描述Thermo Scientific RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues. It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.RNase T1 does not require metal ions for activity. Applications • Removal of RNA from DNA preparations • RNA sequencing • Ribonuclease protection assays. Used in conjunction with RNase A • Removal of RNA from recombinant protein preparations • Determination of the level of RNA transcripts synthesized in vitro from DNA templates containing a 'G-less cassette' |
无参数
微信公众号下单更便捷