描述Thermo Scientific Pierce Nickel Coated Clear 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These clear plates can be used with colorimetric detection methods.Features of Pierce Nickel Coated Plates: • Ni2+ activated surface enables metal-chelate binding of His-tagged proteins • Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene • Better binding for sensitive assays compared to other commercially available nickel-activated plates • Detection limit: 1ng of polyhistidine fusion protein • Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well • Activation level: 200 µL These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. Related Products Pierce™ Nickel Coated Plates, Clear, 8-Well Strip Pierce™ Nickel Coated Plates, White, 96-Well Pierce™ Nickel Coated Plates, Black, 96-Well |
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