描述Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).Highlights • High yields of full-length first strand cDNA up to 13 kb • Optimum activity at 42 to 45°C • Active up to 55°C • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides) Applications • First strand cDNA synthesis for RT-PCR and real-time RT-PCR • Reverse transcription at elevated temperatures to reduce effects of secondary structure • Synthesis of cDNA for cloning and expression • Generation of labeled cDNA probes for microarrays • DNA labeling • Analysis of RNA by primer extension |
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