描述The Thermo Scientific Zeba Dye and Biotin Removal columns enable fast and efficient removal of non-reacted fluorescent dyes, biotin, reducing agents, and crosslinkers from protein samples. Removing free-dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The Zeba Dye and Biotin Removal resin is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Using the appropriate amount of sample and buffer conditions, almost any fluorescent dye can be removed with these columns. Even dilute protein samples (25 µg/mL) can be successfully processed to remove dyes, biotin, reducing agents, and crosslinkers and to efficiently recover proteins (>7 kDa). A single 10-mL Dye and Biotin Removal Spin Column can process a 2 to 4 mL of sample. This product is not recommended for desalting or buffer exchange.Features of Zeba Dye and Biotin Removal columns: • Versatile—removes unreacted fluorescent dyes, biotinylation reagents, crosslinkers, and reducing agents from protein solutions • High recovery—low-binding resin maximizes protein recovery • Easy-to-use—no cumbersome column preparation or equilibration • Fast—small-molecule removal and protein recovery in less than 15 mins • Flexible—available in spin columns and filter spin plates for a range of needs Available formats: • Spin columns: 0.5, 2, 5, and 10 mL • 96-well filter plates: pre-dispensed; compatible with centrifugation for manual or automated purification; enable fast, consistent well-to-well and plate-to-plate reproducibility for small-scale, high-throughput clean-ups Applications: • Ideal for post-reaction clean-up of samples • To effectively remove non-conjugated dye, biotin, reducing agents, and crosslinkers with exceptional protein recovery |
无参数
微信公众号下单更便捷