alpha Tubulin Antibody (14-4502-82) in ICC/IF
Immunofluorescent analysis of alpha tubulin (green) in a BHP 2-7 thyroid cancer anaphase cell cultured in complete medium on a glass slide. Cells were fixed with 4% phosphate-buffered paraformaldehyde for 30 min at 37C, permeabilized with 100% ice-cold methanol for 20 min. and blocked with PBS/1% BSA/0.5% Tween 20 at 37C for 30 min. Cells were stained with monoclonal alpha tubulin antibody (Product # 14-4502-82) at a dilution of 1:200 in PBS/1% BSA/0.1% Tween 20 for 60 min at 37C., and then incubated with goat anti-mouse IgG1 AF488 (Product # A-21121) at a dilution of 1:200 for 60 min. at 37C (panel b: green). HEC1 (panel a: red) was stained simultaneously with monoclonal HEC1 mouse IgG1 antibody (Product # 14-4502-82) followed by goat anti-mouseIgG2a AF594 (1:200, Cat # A-21135). DNA was stained with Hoechst33342 (panel c: blue). Panels d shows a merged image. Images were taken with a 40x immersion oil objective (for more information: Corver et al., Endocr Relat Cancer. 2018 Jan;25(1):83-97). Data courtesy of Antibody Data Exchange Program.
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